A Re-usable Biosensor for Organophosphate Pesticides

Organophosphate Hydrolase in Conductometric Biosensor for the Detection of Organophosphate Pesticides

The research has developed an enzyme biosensor for the detection organophosphate pesticide residues. The biosensor consists of a pair of screen-printed carbon electrode (SPCEs). One of electrodes contains immobilized organophosphate hydrolase (OPH) on a chitosan membrane by cross-linking it with glutaraldehyde. The area of the electrodes was optimized to 3, 5, and 7 mm(2). The OPH was isolated ...

A fluorescence-based biosensor for the detection of organophosphate pesticides and chemical warfare agents

Recently elevated concerns of environmental contamination with organophosphate (OP) insecticides and the possible use of OP nerve agents by terrorists have spurred interest in developing more accurate and sensitive methods for field detection and discrimination of these neurotoxic agents. The analysis of OP neurotoxins typically requires sophisticated equipment, extensive sample preparation tha...

Organophosphate pesticides: A general review

Pesticides are chemicals to control a variety of pests that can damage crops and livestock and reduce farm productivity. Organophosphate (OP) compounds are a group of pesticides that includes some of the most toxic chemicals used in agriculture. OP toxicity is due to the ability of these compounds to inhibit an enzyme, acetyl cholinesterase at cholinergic junctions of the nervous system. This r...

Organophosphorus hydrolase-based assay for organophosphate pesticides

We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphates. This assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which ...

Biosensor for Continuous Monitoring of Organophosphate Aerosols